sonicator probe ultrasonic processor 200h Search Results


dicer  (Santa Cruz Biotechnology)
93
Santa Cruz Biotechnology dicer
Figure 1 | Impaired <t>DICER</t> expression in hypoxic human breast cancers. (a) DICER mRNA abundance in breast cancer patients from METABRIC stratified by DICER copy number and hypoxic fraction as determined with the Winter hypoxia metagene. P-values obtained with Wilcoxon rank sum test. (b) Inverse correlation between DICER mRNA abundance and hypoxia score. P-values obtained with Spearman’s correlation. (c) Kaplan–Meier survival curves for DICER and hypoxia in pooled breast cancer data set consisting of 19 studies. (d) RNA was extracted from indicated breast cancer cell lines exposed to 0.2% O2 for 24 or 48 h and subjected to quantitative reverse transcriptase–PCR analysis of DICER with RPL13A as control (nZ3). Data represent mean±s.e.m. P-values obtained with one-way analysis of variance (ANOVA), Bonferroni’s post-hoc test. (e) Representative western blottings were performed with antibody against DICER and anti-tubulin as control. (f) Densitometric analysis of western blottings in e where the intensity of the DICER bands were normalized for tubulin signal (n ¼ 3). Error bars represent s.e.m. P-values obtained with one-way ANOVA, Bonferroni’s post-hoc test. *Po0.05, **Po0.01, ***Po0.001.
Dicer, supplied by Santa Cruz Biotechnology, used in various techniques. Bioz Stars score: 93/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/sonicator+probe+ultrasonic+processor+200h/Dicer+Antibody/pm25351418-281-11-14
Average 93 stars, based on 1 article reviews
dicer - by Bioz Stars, 2026-11
93/100 stars
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up 200h ultrasonic probe processor  (Hielscher Ultrasonics)
86
Hielscher Ultrasonics up 200h ultrasonic probe processor
Figure 1 | Impaired <t>DICER</t> expression in hypoxic human breast cancers. (a) DICER mRNA abundance in breast cancer patients from METABRIC stratified by DICER copy number and hypoxic fraction as determined with the Winter hypoxia metagene. P-values obtained with Wilcoxon rank sum test. (b) Inverse correlation between DICER mRNA abundance and hypoxia score. P-values obtained with Spearman’s correlation. (c) Kaplan–Meier survival curves for DICER and hypoxia in pooled breast cancer data set consisting of 19 studies. (d) RNA was extracted from indicated breast cancer cell lines exposed to 0.2% O2 for 24 or 48 h and subjected to quantitative reverse transcriptase–PCR analysis of DICER with RPL13A as control (nZ3). Data represent mean±s.e.m. P-values obtained with one-way analysis of variance (ANOVA), Bonferroni’s post-hoc test. (e) Representative western blottings were performed with antibody against DICER and anti-tubulin as control. (f) Densitometric analysis of western blottings in e where the intensity of the DICER bands were normalized for tubulin signal (n ¼ 3). Error bars represent s.e.m. P-values obtained with one-way ANOVA, Bonferroni’s post-hoc test. *Po0.05, **Po0.01, ***Po0.001.
Up 200h Ultrasonic Probe Processor, supplied by Hielscher Ultrasonics, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/sonicator+probe+ultrasonic+processor+200h/processor+ultrasonic+up200st/pm41275462-81-1-7
Average 86 stars, based on 1 article reviews
up 200h ultrasonic probe processor - by Bioz Stars, 2026-11
86/100 stars
  Buy from Supplier
probe ultrasonic  (Hielscher Ultrasonics)
86
Hielscher Ultrasonics probe ultrasonic
Figure 1 | Impaired <t>DICER</t> expression in hypoxic human breast cancers. (a) DICER mRNA abundance in breast cancer patients from METABRIC stratified by DICER copy number and hypoxic fraction as determined with the Winter hypoxia metagene. P-values obtained with Wilcoxon rank sum test. (b) Inverse correlation between DICER mRNA abundance and hypoxia score. P-values obtained with Spearman’s correlation. (c) Kaplan–Meier survival curves for DICER and hypoxia in pooled breast cancer data set consisting of 19 studies. (d) RNA was extracted from indicated breast cancer cell lines exposed to 0.2% O2 for 24 or 48 h and subjected to quantitative reverse transcriptase–PCR analysis of DICER with RPL13A as control (nZ3). Data represent mean±s.e.m. P-values obtained with one-way analysis of variance (ANOVA), Bonferroni’s post-hoc test. (e) Representative western blottings were performed with antibody against DICER and anti-tubulin as control. (f) Densitometric analysis of western blottings in e where the intensity of the DICER bands were normalized for tubulin signal (n ¼ 3). Error bars represent s.e.m. P-values obtained with one-way ANOVA, Bonferroni’s post-hoc test. *Po0.05, **Po0.01, ***Po0.001.
Probe Ultrasonic, supplied by Hielscher Ultrasonics, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/sonicator+probe+ultrasonic+processor+200h/probe+ultrasonic/10__5101_slash_nbe__v13i2__p150___164-68-15-17
Average 86 stars, based on 1 article reviews
probe ultrasonic - by Bioz Stars, 2026-11
86/100 stars
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influenza a h3n2 (a/perth/16/2009) hemagglutinin / ha-specific b cell probe  (Sino Biological)
N/A
A DNA sequence encoding the Influenza A virus (A/Perth/16/2009 (H3N2)) hemagglutinin (ACS71642.1) (Met1-Lys519, with a mutation Tyr 108 Phe), was expressed with a polyhistidine tag at the C-terminus.
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influenza a h3n2 (a-perth-16-2009) hemagglutinin-ha-specific b cell probe protein  (TargetMol)
N/A
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influenza a h3n2 (a/perth/16/2009) hemagglutinin / ha-specific b cell probe insect cell lysate  (Sino Biological)
N/A
Cell lysate was prepared by homogenization of the over-expressed cells in ice-cold modified RIPA Lysis Buffer with cocktail of protease inhibitors (Sigma). Cell debris was removed by centrifugation. Protein concentration was determined by Bradford assay
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Image Search Results


Figure 1 | Impaired DICER expression in hypoxic human breast cancers. (a) DICER mRNA abundance in breast cancer patients from METABRIC stratified by DICER copy number and hypoxic fraction as determined with the Winter hypoxia metagene. P-values obtained with Wilcoxon rank sum test. (b) Inverse correlation between DICER mRNA abundance and hypoxia score. P-values obtained with Spearman’s correlation. (c) Kaplan–Meier survival curves for DICER and hypoxia in pooled breast cancer data set consisting of 19 studies. (d) RNA was extracted from indicated breast cancer cell lines exposed to 0.2% O2 for 24 or 48 h and subjected to quantitative reverse transcriptase–PCR analysis of DICER with RPL13A as control (nZ3). Data represent mean±s.e.m. P-values obtained with one-way analysis of variance (ANOVA), Bonferroni’s post-hoc test. (e) Representative western blottings were performed with antibody against DICER and anti-tubulin as control. (f) Densitometric analysis of western blottings in e where the intensity of the DICER bands were normalized for tubulin signal (n ¼ 3). Error bars represent s.e.m. P-values obtained with one-way ANOVA, Bonferroni’s post-hoc test. *Po0.05, **Po0.01, ***Po0.001.

Journal: Nature communications

Article Title: Hypoxia promotes stem cell phenotypes and poor prognosis through epigenetic regulation of DICER.

doi: 10.1038/ncomms6203

Figure Lengend Snippet: Figure 1 | Impaired DICER expression in hypoxic human breast cancers. (a) DICER mRNA abundance in breast cancer patients from METABRIC stratified by DICER copy number and hypoxic fraction as determined with the Winter hypoxia metagene. P-values obtained with Wilcoxon rank sum test. (b) Inverse correlation between DICER mRNA abundance and hypoxia score. P-values obtained with Spearman’s correlation. (c) Kaplan–Meier survival curves for DICER and hypoxia in pooled breast cancer data set consisting of 19 studies. (d) RNA was extracted from indicated breast cancer cell lines exposed to 0.2% O2 for 24 or 48 h and subjected to quantitative reverse transcriptase–PCR analysis of DICER with RPL13A as control (nZ3). Data represent mean±s.e.m. P-values obtained with one-way analysis of variance (ANOVA), Bonferroni’s post-hoc test. (e) Representative western blottings were performed with antibody against DICER and anti-tubulin as control. (f) Densitometric analysis of western blottings in e where the intensity of the DICER bands were normalized for tubulin signal (n ¼ 3). Error bars represent s.e.m. P-values obtained with one-way ANOVA, Bonferroni’s post-hoc test. *Po0.05, **Po0.01, ***Po0.001.

Article Snippet: Membranes were probed overnight at 4 C with antibodies directed against: DICER (1:200, H-212; Santa Cruz Biotechnology), CA9 (1:1,000, M75; gift from Dr Silvia Pastorekova), H3K27me3 (1:20,000, 07-449; Upstate), H3 (1:5,000, D1H2; Cell Signaling Technology), E-cadherin (1:1,000, Cell Signaling Technology), Vimentin (1:500, clone V9; Sigma), b-tubulin (1:20,000, Abcam), b-actin (1:20,000, Sigma) or eIF4E (1:1,000, BD Transduction Laboratories).

Techniques: Expressing, Reverse Transcription, Control, Western Blot

Figure 5 | Hypoxia stimulates cancer stem cell-associated phenotypes in a miR-200b-dependent manner. HMLER cells overexpressing miR-200b or green fluorescent protein (GFP) (n ¼ 3) grown for 7 days under 1% O2 or control conditions. (a,b) quantitative reverse transcriptase–PCR analysis was performed to measure (a) mature miR-200b levels and (b) DICER, ZEB1 and E-cadherin mRNA levels where RPL13A served as control (n ¼ 3). (c) Representative FACS analysis using antibodies specific for CD44 and CD24. (d) Quantification of the percentage of cells with CD44 highCD24low for three independent experiments. (e) Sphere formation assay, number spheres formed per 500 cells plotted (n ¼ 3). (f) Model of hypoxia-mediated EMT and stemness. Error bars represent s.e.m. P-values obtained with Student’s t-test. *Po0.05, **Po0.01.

Journal: Nature communications

Article Title: Hypoxia promotes stem cell phenotypes and poor prognosis through epigenetic regulation of DICER.

doi: 10.1038/ncomms6203

Figure Lengend Snippet: Figure 5 | Hypoxia stimulates cancer stem cell-associated phenotypes in a miR-200b-dependent manner. HMLER cells overexpressing miR-200b or green fluorescent protein (GFP) (n ¼ 3) grown for 7 days under 1% O2 or control conditions. (a,b) quantitative reverse transcriptase–PCR analysis was performed to measure (a) mature miR-200b levels and (b) DICER, ZEB1 and E-cadherin mRNA levels where RPL13A served as control (n ¼ 3). (c) Representative FACS analysis using antibodies specific for CD44 and CD24. (d) Quantification of the percentage of cells with CD44 highCD24low for three independent experiments. (e) Sphere formation assay, number spheres formed per 500 cells plotted (n ¼ 3). (f) Model of hypoxia-mediated EMT and stemness. Error bars represent s.e.m. P-values obtained with Student’s t-test. *Po0.05, **Po0.01.

Article Snippet: Membranes were probed overnight at 4 C with antibodies directed against: DICER (1:200, H-212; Santa Cruz Biotechnology), CA9 (1:1,000, M75; gift from Dr Silvia Pastorekova), H3K27me3 (1:20,000, 07-449; Upstate), H3 (1:5,000, D1H2; Cell Signaling Technology), E-cadherin (1:1,000, Cell Signaling Technology), Vimentin (1:500, clone V9; Sigma), b-tubulin (1:20,000, Abcam), b-actin (1:20,000, Sigma) or eIF4E (1:1,000, BD Transduction Laboratories).

Techniques: Control, Reverse Transcription, Tube Formation Assay